Reprod Dev Med · 2025 · RCT Protocol · China

Focused Ultrasound for Cervical Lesions with Fertility Preservation — Study Protocol

聚焦超声治疗宫颈病变并保留生育力——研究方案
Randomized controlled trial · 2-year follow-up · CIN2/3 with TZ 1–2, family planning needed · ChiCTR2300076739
Yang et al. · Third Affiliated Hospital of Zhengzhou University
Reproductive and Developmental Medicine · doi: 10.1097/RD9.0000000000000132
96.8%
efficacy at 3–6 mo (prior data)
2.0%
recurrence 6–12 mo
72.4→81%
HPV clearance
24 mo
follow-up
Fertility
preservation goal

1Background & Objective

  • Dilemma: CIN2+ in childbearing women — excision (LEEP/CKC) is effective but risks cervical insufficiency, preterm birth & delayed fertility.
  • FUS: non-ionizing, non-invasive focused ultrasound — heats lesion >60°C, coagulates without harming surrounding tissue.
  • Objective: evaluate FUS efficacy & fertility protection for cervical lesions in women needing family planning.

2Study Design

RCT
2-year follow-up
CIN2/3
TZ 1–2
ChiCTR
2300076739
FUS treatmentvs standard care
  • Setting: Third Affiliated Hospital of Zhengzhou University; ethics 2023-001.
  • Inclusion: >18 y, histologic CIN2/CIN3 (TZ 1–2), family planning required.
  • Exclusion: pregnancy/lactation · prior conization/LEEP/CKC · vaginal lavage <24 h · acute inflammation.
  • Endpoints: HPV clearance, lesion regression, pregnancy & delivery outcomes, safety.
  • Treatment: FUS session per protocol; quality control; data by dedicated physician.
  • Data: full exam + psychological scale + recent records at each visit.
  • Analysis: ITT + per-protocol; time-to-event for pregnancy outcomes.
3·6·12
month visits
18·24
month visits
8
scheduled visits
Focus >60°C Coagulation Lesion cleared
Outpatient· No excision· Fertility spared
FUS: single outpatient session, no cervical tissue removed.

3Follow-Up Schedule — 24 Months

BaselineFUS / exams
3 mo
6 mo
12 mo
18 mo
24 mopregnancy / delivery
  • Visits: HPV, TCT, colposcopy, pathology, psychological scale at each timepoint.
  • Fertility: pregnancy attempt after lesion regression; cervical biopsy before pregnancy & after delivery.

4Treatment Options for CIN2–3

MethodEfficacyFertility impactNotes
FUS (this trial)96.8% (prior)non-invasiveno tissue removal
LEEP / CKCeffectivecervical insufficiency riskpreterm birth risk
Cryotherapyeffectivefewer pregnancy riskslarge discharge; N2/CO2 supply
Prior FUS reports: CIN1 efficacy ~90%, HPV clearance 85.7%; Zeng 2021 (n=154): efficacy 96.8%, recurrence 2.0%.

5Expected Value

96.8%
efficacy 3–6 mo
2.0%
recurrence
81.0%
HPV clearance
Efficacy
96.8%
HPV clearance
81%
Recurrence
2.0%
  • Fertility preservation: no excision → lower preterm birth & cervical insufficiency risk.
  • Efficacy: high lesion regression and HPV clearance in earlier series.
  • Evidence gap: RCT-level data for childbearing women with CIN2/3 — this trial will provide it.
  • Safety: no severe adverse reactions reported in prior FUS series.
  • Established use: FUS already treats cervicitis & vulvovaginal disease.
Clinical Significance